“Pick up the pace, ladies!”
You coach a kids' soccer team. The players are practicing, but they're not running as fast as they can. You want them to run faster, so you say this.
Pick up the pace, ladies!
To "pick up the pace" means to go faster. You can use this to talk about running, working, reading, and other things. However, you probably wouldn't use "pick up the pace" to talk about the speed of a car.
You can call a group of girls or women "ladies". It's common in a situation like the one above: when a teacher or coach is talking to a group of girls or women. It sounds polite, but authoritative.